Effects of Parathyroid Hormone on Immune Function

Table 3

Study discussing the effect of PTH on B-lymphocytes.

	Study design	PTH	Assays	Results
Alexiewicz et al. [38]	B cells: 21 hemodialysis patients, 37 healthy subjects Cultured for 5 days.	Bovine (1–84) PTH, Purified (1–34) PTH, (1–84) PTH, Inactivated (1–84) PTH.	Stimulation with S. aureus cowan strain I	(i) Lower proliferation potential for B-lymphocytes of hemodialysis patients.; (ii) (1–34) and (1–84) PTH produced dose-dependant inhibition of proliferation.; (iii) (1–84) inhibitory effect was more than (1–34).
		1×10^−7,2×10^−7and4×10^−7 M	Proliferation assessed with thymidine uptake.
Gaciong et al. [51]	Mononuclears cells: 34 hemodialysis patients,; 44 healthy subjects; Cultured 8 days.	Bovine (1–84) PTH, bovine (1–34) PTH. PTH was added at the beginning and at day 2.	Stimulation with S. aureus cowan strain I	(i) (1–84) and (1–34) PTH inhibited immunoglobulin production.; (ii) At the lower dose of (1–84) PTH had no inhibition of B lymphocytes of ESRD patients.; (iii) Inactivated (1–84) had no inhibitory effect.; (iv) The inhibition was only observed when PTH was added at the beginning of the culture.
		5×10^−7and 10^−6 M	Function was assessed by Ig production.
Jiang et al. [55]	Human B-cell lines (CBL3, SKW6.4, CESS).; Incubated for 5 days	Human (1–84) PTH	Stimulation with S. aureus cowan strain I	(i) PTH directly inhibits Ig production by B lymphocytes.; (ii) PTH did not affect B-cell growth; (iii) IL-4 reduced the inhibitory effect of PTH on Ig production.
		0.01, 0.1, 1, 10, 100 ng/ml.	Proliferation assessed with thymidine uptake.