Long-Term Follow-Up of HLA-A2+ Patients with High-Risk, Hormone-Sensitive Prostate Cancer Vaccinated with the Prostate Specific Antigen Peptide Homologue (PSA146-154)
Figure 1
A representative flow cytometric data showing the detection of CD8+ PSA146-154 peptide-tetramer+ cells in patient UPIN28. PBMC were sensitized in vitro with PSA146-154 peptide for 3 cycles, and resulting T-cells were doubly stained with PSA146-154 peptide-tetramer-PE ((c) and (d)) or negative control tetramer-PE ((e) and (f)) and CD8-FITC ((e), (f), (c), and (d)). A greater number of CD8+ PSA146-154 peptide-tetramer+ cells ((a) and (b)) were observed on postvaccine compared to prevaccine samples.
