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Clinical and Developmental Immunology
Volume 2010, Article ID 537531, 10 pages
Research Article

Clinicopathologic Significance of HIF-1 , CXCR4, and VEGF Expression in Colon Cancer

1Department of Surgery, The First People Hospital of Changzhou and The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu 213000, China
2Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences and Shanghai Jiao Tong University School of Medicine, Chinese Academy of Sciences, Shanghai 200025, China
3Department of Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, China

Received 28 June 2010; Revised 26 August 2010; Accepted 15 September 2010

Academic Editor: Silvano Sozzani

Copyright © 2010 Yugang Wu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We investigated the clinicopathologic significance of HIF-1, CXCR4, and VEGF expression using immumohistochemistry in human colon cancer. HIF-1, CXCR4, and VEGF high expression levels were correlated positively with TNM stage, lymph node involvement, and distant metastasis Furthermore, we found that combined high expression of any two of the three molecules ( for HIF-1/CXCR4, for HIF-1/VEGF, and for CXCR4/VEGF) had stronger correlation with lymph node metastasis than did each alone. However, a relationship with distant metastasis is seen only with the combinations CXCR4/VEGF ( for HIF-1/CXCR4, for HIF-1/VEGF, and for CXCR4/VEGF) as compared with those of single molecule high expression alone. Combined expression of all three molecules strongly correlates with lymph node metastasis and distant metastasis. The mRNA expression of HIF-1, CXCR4, and VEGF were quantified by real-time PCR in different colon cancer tissue samples, the experiment results shown that fresh colon tissue samples significantly overexpressed CXCR4 and VEGF mRNA compared with negative control. Therefore, the disease-free survival of all patients after curative resection can be considered in association with all three markers expression.