Cytokine Production by Peripheral Blood CD4+ and CD8+ T Cells in Atopic Childhood Asthma
Figure 2
(a) Flow cytometry of the cytokine production values of IL-2, IL-4, IL-10, IL-13, INF-γ, and TNF-α in CD4+ and CD8+ T subpopulations. Example of one patient with mild asthma and one control. (b) Plots of CD4+ and CD8+ T cells producing various cytokines before and after polyclonal stimulation of one patient with mild asthma (male, age 9.8 yrs) and one control (male, age-9.5 yrs). B-baseline, spontaneous cytokine staining without stimulation (in the presence of monensin alone), S-cytokine staining with stimulation (unseparated whole blood from asthma patients and healthy controls was stimulated for 4 h with PMA and ionomycin, and production of IL-2, IL-4, IL-10, IL-13, INF-γ, and TNF-α of CD4+ and CD8 T+ cells was determined at the single cell levels by intracellular cytokine staining and flow cytometry as described in Section 2).
