Research Article

Systemic Administration of CpG Oligodeoxynucleotide and Levamisole as Adjuvants for Gene-Gun-Delivered Antitumor DNA Vaccines

Figure 2

Detection of E7-specific splenic CTLs after DNA vaccination combined with systemic administration of adjuvants. C57BL/6 mice ( ) were twice immunized at a 1-week interval with 1 μg of pBSC or pBSC/E7GGG. GUS by a gene gun and i.p. injected with indicated doses of CpG-ODN (a) or LMS (b). One week after the second immunization, lymphocyte bulk cultures were prepared from splenocytes, restimulated with the RAHYNIVTF peptide for 6 days, and stained with a mixture of H-2Db/E749-57-PE tetramers and anti-mouse CD8a-FITC antibody. Control lymphocytes were cultured without the peptide. Columns: mean of duplicate samples; bars: ±SD; * (the comparison with the E7GGG.GUS group).
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(a)
176759.fig.002b
(b)