Patients with Multiple Myeloma Develop SOX2-Specific Autoantibodies after Allogeneic Stem Cell Transplantation
Figure 3
(a) Analysis of specificity of the SOX2 targeted IgG antibody response. Serum from a patient found positive by ELISA was used for western blot analysis and specifically recognized recombinant SOX2 and SOX2 from a SOX2-positive cell line (U266). In contrast, GST and a SOX2-negative cell line (DLD1) remained unstained. ACTB was used as loading control. (b) Mapping of the epitopes of target of the SOX2-specific antibody response in MM patients. Overlapping 20 mer peptides () spanning the complete SOX2 sequence were used. Percentages of SOX2-antibody-positive patients for each epitope are given on the -axis. Three patients recognized two or three epitopes. 8 patients had SOX2-specific antibodies only directed against the 20 mer 22 (amino acids 211–230), and the antibodies of five patients did not recognize any of the 20 mers that were used. (c) Epitope prediction of the antibody response for the whole SOX2 protein sequence using a hidden Markov prediction model. For each region probability scores are calculated. The grey area represents the main 20 mers of target by the SOX2 antibody response in MM patients.
