Research Article

IFN Regulatory Factors 4 and 8 Expression in the NOD Mouse

Figure 3

Expression of IRF4 in splenic DCs of diabetes-prone NOD mice and diabetes-resistant BALB/c mice. Proteins were extracted from CD11c+-purified splenic DCs from NOD and BALB/c mice and analyzed by Western blotting for (a) IRF4 or (b) IRF8 expression. The relative intensities of the bands were assessed using the NIH Image software and normalized to reference actin bands to establish a ratio of IRF4/actin (a), lower panel) and (b), lower panel) IRF8/actin. The expression levels observed in BALB/c were arbitrarily set as a unitary value. Data are representative of 2 independent experiments (Exp1 and Exp2). (c) and (d) splenocytes from NOD and BALB/c mice were stained for the CD11c, CD4, and CD8α surface markers in combination with intracellular staining with an anti-IRF8 or anti-IRF4 mAb and analyzed by flow cytometry. Data represent the average percentage of CD11c+CD4+CD8α IRF4+-positive (c) and CD11c+CD4CD8α +IRF8+-positive (d) cells of two independent experiments. Error bars correspond to the averages ±S.D (* , ** and *** ).
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