Upregulated Expression of Indoleamine 2, 3-Dioxygenase in Primary Breast Cancer Correlates with Increase of Infiltrated Regulatory T Cells In Situ and Lymph Node Metastasis
Figure 3
The proportion and absolute number of CD4+CD25+CD127− Tregs in CD3+ T cells increased after coculture with IDO/CHO cells. CD3+ T cells isolated from PBMCs of breast cancer patients were cocultured with CHO/IDO or CHO/EGFP cells for 7 days to allow induction of Tregs. The proportions and absolute number of CD4+CD25+CD127− Tregs were detected by flow cytometry. (a) The proportion of CD4+CD25+CD127− Tregs in the T cells treated with IDO− CHO/EGFP cells (P2 region represents CD4+ T cells; Q4 region represents CD4+CD25+CD127− Tregs). (b) The proportion of CD4+CD25+CD127− Tregs in the T cells treated with IDO+ CHO/IDO cells (P2 region represents CD4+ T cells; Q4 region represents CD4+CD25+CD127− Tregs). (c) The CD3+ control T cells (P5 region represents CD4+ T cells; Q4 region represents CD4+CD25+CD127− Tregs). The flow cytometry dot plots indicate data of one representative experiment. Each experiment was repeated at least 3 times.
