Newborn Mice Vaccination with BCG.HIVA222 + MVA.HIVA Enhances HIV-1-Specific Immune Responses: Influence of Age and Immunization Routes
Expression of the HIVA immunogen from BCG.HIVA222. (a) A synthetic GC-rich HIVA gene consisting of consensus was fused to the 19-kDA lipoprotein signal sequence and inserted into episomal pJH222 E. coli-mycobacterium shuttle plasmid. This contains a kanamycin resistance gene (aph) and complementing lysA genes and E. coli origin of replication (oriE). In addition, pJH222 contains mycobacterial origin of replication (oriM). BALB/c mice T-cell and mAb Pk epitopes used in this paper are depicted. Pα-Ag, M. tuberculosisα-antigen promoter; PHSP60, heat shock protein 60 gene promoter. (b) Western blotting of lysates of BCG.HIVA222 containing the pJH222HIVA (lanes 1 and 2) and BCG wild type (lane 3; negative control) is shown. HIVA was detected using the anti-Pk mAb followed by HRP-protein A and ECL.
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