Newborn Mice Vaccination with BCG.HIVA222 + MVA.HIVA Enhances HIV-1-Specific Immune Responses: Influence of Age and Immunization Routes
Effect of BCG.HIVA222 priming on the induction of HIV-1-specific CD8+ T cells. (a) Mice were immunized with cfu (adult mice I.D. or neonates S.C.) or 106 cfu (adult mice S.C.) of BCG.HIVA222 and subsequently boosted 14 weeks later with 106 pfu of MVA.HIVA by i.m. route. (b and c) Analysis of IFN-γ and TNF-α vaccine-elicited CD8+ T cells as generated for each vaccination group by using the P18I10 epitope. The frequencies of CD8+ T cells producing IFN-γ or TNF-α are shown. Data are presented as means ± standard deviation (SD; ). (d) Elicitation of specific T-cell responses was assessed in an IFN-γ ELISPOT assay using the immunodominant P18I10 CD8+ T-cell epitope peptide. The mean (± SEM) sfu per 106 splenocytes for each group of mice ( per group) is shown. BCG.HIVA222 primed mice: striped bars. BCGwt primed mice: white solid bars. ; , .
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