The M. tuberculosis Phosphate-Binding Lipoproteins PstS1 and PstS3 Induce Th1 and Th17 Responses That Are Not Associated with Protection against M. tuberculosis Infection
Figure 6
PstS1-specific IL-17—but not IFN-γ—secreting cells were generated during M. tuberculosis infection. Naïve unvaccinated or BCG-vaccinated mice (5 mice/group) were infected or not with M. tuberculosis for 4 or 11 weeks. Spleen cells recovered from mice were stimulated ex vivo ( cells/well) with PstS1, PstS3, or Ag85B proteins (5 μg/mL each) for 4 days and culture cell supernatants assayed for IFN-γ (a) or IL-17 (b) by specific ELISA kits. Data were combined from 3 independent experiments and are presented as mean. Error bars indicate SEM.