Evaluation of Epstein-Barr Virus Latent Membrane Protein 2 Specific T-Cell Receptors Driven by T-Cell Specific Promoters Using Lentiviral Vector
Figure 5
Significantly reduced tumor burden and enhanced tumor-free survival in nude mice implanted with LMP2-expressing tumors after infused with transduced T cells. (a) Tumor mouse model was established as described. After 7 days, mice were infused with different lentiviral transduced T cells twice at a 1-week interval. PBS-immunized mice were used as control. Tumor growth was recorded twice a week. Tumor sizes are expressed as the average of two perpendicular diameters of the tumor. Graphs show mean ± SE; *; /group. (b) Comparison of survival times of tumor-bearing mice infused with T cells ex vivo transduced by lentivirus containing LMP2-TCR α and β chains driven by various T-cell promoters. Tumor burden was monitored on a weekly basis. Significant differences were observed for all transduced T-cell vaccination group. *; /group. Note: tumor growth rates and survival times were discontinued when the tumor reached 1 cm2 and the mouse was sacrificed.
