Comparative Approach to Define Increased Regulatory T Cells in Different Cancer Subtypes by Combined Assessment of CD127 and FOXP3

Figure 5

Assessment of naïve CCR7⁺CD45RA⁺CD4⁺CD127^lowFOXP3⁺ T_reg cells. (a) Frequencies of CCR7⁺CD45RA⁺ naïve CD4⁺CD127^lowFOXP3⁺ T_reg cells (T_naive), CCR7⁺CD45RA⁻CD4⁺CD127^lowFOXP3⁺ central memory T_reg cells (T_CM), and CCR7⁻CD45RA⁻CD4⁺CD127^lowFOXP3⁺ effector memory T_reg cells (T_EM) were assessed in peripheral blood using gating on CD127 and FOXP3 with successive gating on CCR7 and CD45RA. (b) Flow cytometric analysis of naïve, central memory, and effector memory CD4⁺CD127^lowFOXP3⁺ T_reg cells in peripheral blood from a representative healthy individual (control) as well as representative patients with colorectal cancer (CRC), CLL, MGUS, and multiple myeloma (MM). Frequencies of regulatory T_naive, T_CM, and T_EM cells were assessed in (c) peripheral blood of CRC (CRC, 𝑛=12), CLL (CLL, 𝑛=10), MGUS (MGUS, 𝑛=7), MM (MM, 𝑛=10), and healthy individuals (control, 𝑛=10) and (d) 7 reactive lymph node biopsies from healthy individuals (reactive), 7 patients with follicular lymphoma (FL), and 6 patients with Hodgkin’s disease (HD). Error bars represent standard deviation (*, 𝑃<0.05, Student’s t-test).

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