Different Transcriptional Profiles of Human Monocyte-Derived Dendritic Cells Infected with Distinct Strains of Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-Guérin

Table 2

(a) Upregulated genes of DCs infected with different MTB and BCG strains. (b) Downregulated genes of DCs infected with different MTB and BCG strains.

(a)

Gene	MTB H37Rv	MTB CMT97	BCG Aventis	BCG Japan	Common name	Classification	Function
ADAM19	+	+	+	+		Metalloproteinase	Migration/Inflammation
BASP1	+	+	+	+	CAP-23, NAP-2	Cell surface protein	Prot-prot interaction
CCL5	+	+	+	+	RANTES	Chemokine ligand	Migration
CCL20	+	+	+	+	MIP-3α	Chemokine ligand	Inflammation
CCL22	+	+	+	+	MDC	Chemokine ligand	Inflammation
CCR7	+	+	+	+		Chemokine receptor	Migration
CD83	+	+	+	+		Cell surface protein	Ag presentation
IL-1β	+	+	+	+		Cytokine	Inflammation
IL-6	+	+	+	+		Cytokine	Inflammation
IL-10	+	+	+	+		Cytokine	Immune regulation
IL-12p40	+	+	+	+		Cytokine	T cell stimulation
LY75	+	+	+	+	DEC-205	Cell surface receptor	Ag presentation
NFKB1	+	+	+	+		Trascriptional factor	Signal transduction
SOD2	+	+	+	+		Mytocondrial protein	Oxidative stress
TAP2	+	+	+	+		Ag transporter 2	Ag presentation
TLR2	+	+	+	+		Toll-like receptor	Pathogen assoc. receptor
TNF-α	+	+	+	+		Cytokine	Inflammation
ADAR	+			+		Adenosine deaminasi	RNA modification
CCL3	+	+	+		MIP-1α	Chemokine ligand	Inflammation
CCL19			+	+		Cell surface receptor	Migration
CD80				+		Cell surface protein	Ag presentation
CD86		+	+	+		Cell surface protein	Ag presentation
CFLAR			+	+	C-Flip	Apoptosis regulator	Apoptosis inhibitor
CRF			+			C1q related factor
EBI3	+	+				Secreted glicoprotein	Immune-regulation
IL-1a		+	+	+		Cytokine	Inflammation
LAMP3	+	+		+	CD63	Lysosomal associated protein	Ag capture
MT2A		+	+			Metallothionein	Oxidative stress
PLAUR		+	+		CD87	Cell surface receptor	Migration
TNFRSF6			+	+	CD95, FAS	Cell surface receptor	Maturation

(b)

Gene	MTB H37Rv	MTB CMT97	BCG Aventis	BCG Japan	Common name	Classification	Function
ARHGDIB	−	−	−	−		RhoGDP dissociation inhibitor 2	Cell motility and adhesion
CD1A	−	−	−	−		MHC I like protein	Ag presentation
CD1B	−	−	−	−		MHC I like protein	Ag presentation
CD1C	−	−	−	−		MHC I like protein	Ag presentation
CD36	−	−	−	−		Cell surface protein	Ag capture
CD68	−	−	−	−		Cell surface protein	Ag capture
CD74	−	−	−	−	Invariant chain	MHC II assoc. protein	Ag loading
CD209	−	−	−	−	DC-SIGN	C-type lectin receptor	T/DCs interaction
CLECSF12	−	−	−	−	DECTIN-1	Cell surface receptor	Pattern recog. receptor
CLECSF6	−	−	−	−	DCIR	C-type lectin receptor	Ag capture
CST3	−	−	−	−		Proteinase inhibitor
CXCL16	−	−	−	−		Chemokine	T cell stimulation
FCER1A	−	−	−	−		Fc receptor	Inflammation
FCER2	−	−	−	−		Fc receptor	Inflammation
GIP3	−	−	−	−		IFN-α inducible protein
HLA-DMA	−	−	−	−		MHC II accessory protein	Ag loading
HLA-DMB	−	−	−	−		MHC II accessory protein	Ag loading
IFI16	−	−	−	−		IFN-γ inducible protein	Cell cycle regulator
IFITM3	−	−	−	−		IFN inducible protein	Immune response
ITGB2	−	−	−	−		Integrin	Migration
LANGERIN	−	−	−	−		Cell surface protein	Ag capture
LIPA	−	−	−	−	Lipase A	Acid lipase	Lipidic metabolism
MX1	−	−	−	−		IFN-α inducible p78	Antiviral response
RNASE6	−	−	−	−		Ribonuclease	Ag presentation
TLR4	−	−	−	−		Toll-like receptor	Pathogen assoc. receptor
CSF1R	−	−		−	CD115	Cell surface receptor	M-CSF receptor
DCP1B	−			−		Decapping enzyme hDcp1b	RNA degradation
DCSTAMP	−		−			Cell trasmemb. protein
GBP3				−		Guanilate binding protein
GIP2	−			−		IFN-α inducible protein
IL-18				−		Cytokine	Inflammation
PFN1				−
TLR6	−			−		Toll-like receptor	Pathogen assoc. receptor

Upregulated (Table 2(a)) and downregulated (Table 2(b)) genes of DCs exposed for 3 h to MTB H37Rv, MTB CMT97, BCG Aventis and BCG Japan as compared to uninfected DCs were assayed by macroarray 1-day culture after infections. For genes to be referred as upregulated or downregulated we considered only those that showed at least a twofold change in the level of RNA as compared to uninfected DCs expression, in at least 2 independent experiments of the 3 performed.