Lactobacillus paracasei Reduces Intestinal Inflammation in Adoptive Transfer Mouse Model of Experimental Colitis

Figure 5

Phenotypic characterization of mesenteric DC after colitis induction. 5 × 10⁵ sorted naïve T cells were adoptively transferred into Rag2^−/− mice. (a) After 4 weeks, mesenteric lymph node cell suspensions were made, stained with anti-CD11c and anti-CD11b mAbs, and analyzed by flow cytometry. DC populations were equally important in colitic PBS-fed recipient mice (AT) and ST11-fed mice (AT and ST11-fed). As control, representative stainings obtained with unmanipulated C57BL/6 wild-type (WT) or Rag2^−/− mice (Rag2^−/−) are shown. Among DCs, two different subsets were obtained: CD11b^−/lowCD11c⁺ and CD11b⁺CD11c⁺ DCs. (b) Representative staining showing CD40, CD80, and CD86 expressions on gated CD11b^−/lowCD11c⁺ (empty histograms) or CD11b⁺CD11c⁺ DCs (filled histograms) are shown. (c) Shown is a correlation curve between ratio of CD11b^−/lowCD11c⁺ and CD11b⁺CD11c⁺ DCs and body weight change in % loss (negative values) or gain (positive values) in colitic or healthy mice. Each symbol represents an individual mouse. Black-filled, red-filled, or empty squares were colitic PBS-fed mice, colitic ST11-fed mice, or healthy Rag2^−/− mice, respectively.

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