Protection against Autoimmune Diabetes by Silkworm-Produced GFP-Tagged CTB-Insulin Fusion Protein

Figure 1

Schematic structure of the CTB-Ins-GFP fusion gene. AcMNPV Ph-Pro: Autographa californica multiple nuclear polyhedrosis virus polyhedrin promoter, CTB: cholera toxin B subunit, L: linker peptide (GPGP), InsA: human insulin subunit A, InsB: human insulin subunit B (InsA and InsB are linked by mini-C peptide (RRGSKR)), GFP: green fluorescent protein, and LB and RB: left and right border, respectively, of the donor vector pFastBac1.