Figure 5: Contribution of the RP S19 oligomers to the K562-THP-1 cell interaction (a) Time schedule for the K562 cell that were cocultured with the THP-1 cells. (b) K562 cells expressing RFP-Nucleus/EGFP-ANXA3 were cultured for 9 days after the haemin induction and were examined under fluorescence microscopy. The microscopic images (4 pairs) were used to count the number of K562 cells on each THP-1 macrophage-like cell using the Image-J software ( ). values of less than 0.05 were considered statistically significant (* ).