Prediction and Identification of Potential Immunodominant Epitopes in Glycoproteins B, C, E, G, and I of Herpes Simplex Virus Type 2

Table 2

Antigenicity and specificity analysis of the five epitopes using EIA.

Number	Antigen (peptides-BSA)	Antibody to the proteins (titer)	OD value (450 nm)	Antigen (proteins)	Antibody to peptides-BSA (titer)	EIA A450 value
(1)	gB2466–473/BSA	gB2; (1 : 10 000)	0.977±0.039	gB2	gB2466–473/BSA (1 : 5000)	1.099±0.037
(2)	gC2216–223/BSA	gC2; (1 : 10 000)	0.899±0.031	gC2	gC2216–223/BSA (1 : 5000)	1.102±0.040
(3)	gE2483–491/BSA	gE2; (1 : 10 000)	1.102±0.040	gE2	gE2483–491/BSA (1 : 5000)	0.893±0.029
(4)	gG2572–579/BSA	gG2; (1 : 10 000)	0.882±0.011	gG2	gG2572–579/BSA (1 : 5000)	0.986±0.031
(5)	gI2286–295/BSA	gI2; (1 : 10 000)	0.792±0.029	gI2	gI2286–295/BSA (1 : 5000)	0.876±0.031
(6)	BSA	All the proteins^# (1 : 10 000)	<0.2000	All the proteins	BSA** (1 : 5000)	<0.2000

^#: BSA did not react with all the antisera to the five proteins.
**: The five proteins did not react with all the antisera to BSA.