Pulmonary Immune-Compartment-Specific Interferon Gamma Responses in HIV-Infected Individuals with Active Tuberculosis (TB) in an Area of High TB Prevalence
Figure 1
Flow cytometry dot plots showing gamma interferon production from the total sputum lymphocyte population. (a) shows exclusion of doublets by gating cells with proportional forward scatter area (-axis) against forward scatter height (-axis). (b) shows gating of total lymphocytes according to forward scatter (-axis) and side scatter (-axis). (c) shows the gamma interferon produced from total lymphocytes after stimulation with PPD. The position of the gate was determined in the unstimulated control tube (d) and copied into the antigen stimulated tubes.
