Pulmonary Immune-Compartment-Specific Interferon Gamma Responses in HIV-Infected Individuals with Active Tuberculosis (TB) in an Area of High TB Prevalence
Figure 2
Flow cytometry dot plots showing gamma interferon production from the CD27− CD4+ memory T lymphocyte population. (a) shows exclusion of doublets by gating cells with proportional forward scatter area (-axis) against forward scatter height (-axis). (b) shows gating of total lymphocytes according to forward scatter (-axis) and side scatter (-axis). (c) shows the CD3+ and CD4+ coexpressing lymphocytes. (d) comprises only CD3+ CD4+ lymphocytes and shows the CD27− (CD27 on x-axis and SSC on y-axis) CD3+ CD4+ lymphocytes. (e) shows the percentage of IFNγ secreting memory CD27− CD4+ memory T lymphocytes. The position of the gate was determined in the unstimulated control tube and copied into the stimulated tubes.
