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Clinical and Developmental Immunology
Volume 2012, Article ID 538701, 9 pages
http://dx.doi.org/10.1155/2012/538701
Research Article

Human Antibodies Can Cross Guinea Pig Placenta and Bind Its Neonatal Fc Receptor: Implications for Studying Immune Prophylaxis and Therapy during Pregnancy

1Laboratory of Plasma Derivatives, Division of Hematology, OBRR, FDA, Bethesda, MD 20892, USA
2Division of Veterinary Services, Center for Biologics Evaluation and Research, FDA, Bethesda, MD 20892, USA

Received 8 May 2012; Revised 10 July 2012; Accepted 11 July 2012

Academic Editor: K. Blaser

Copyright © 2012 Evi Budo Struble et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Despite increased use of monoclonal and polyclonal antibody therapies, including during pregnancy, there is little data on appropriate animal models that could humanely be used to understand determinants of protection and to evaluate safety of these biologics in the mother and the developing fetus. Here, we demonstrate that pregnant guinea pigs can transport human IgG transplacentally at the end of pregnancy. We also observe that human IgG binds to an engineered soluble variant of the guinea pig neonatal Fc receptor in vitro in a manner similar to that demonstrated for the human variant, suggesting that this transplacental transport mirrors the receptor-based mechanism seen in humans. Using an intravenous antihepatitis B-specific immune globulin preparation as an example, we show that this transport results in neutralizing activity in the mother and the newborn that would potentially be prophylactic against hepatitis B viral infection. These preliminary data lay the groundwork for introducing pregnant guinea pigs as an appropriate model for the evaluation of antibody therapies and advancing the health of women and neonates.