TCR Gene Transfer: MAGE-C2/HLA-A2 and MAGE-A3/HLA-DP4 Epitopes as Melanoma-Specific Immune Targets
Figure 2
Primary human T cells transduced with TCR-Vα3/Vβ28 genes bind MC2/A2 pMHC. The MC2/A2 TCR T cells were labeled with PE-conjugated pentamers before flow cytometric analysis (solid lines). (a) T cells transduced either with TCR-Vα2Cα/Vβ28Cβ1 and Vα3Cα/Vβ28Cβ1 or control TCRαβ genes (Mock), and not sorted for MC2/A2 binding. (b) T cells transduced with TCR-Vα3Cα/Vβ28Cβ1 genes and FAC Sorted with MC2/A2 pentamer. Results are from a representative transduction out of 6 transductions of PBMC from 2 donors with similar results.
