TCR Gene Transfer: MAGE-C2/HLA-A2 and MAGE-A3/HLA-DP4 Epitopes as Melanoma-Specific Immune Targets
Figure 5
MA3/DP4 TCR T cells specifically lyse MA3-transduced or peptide-loaded B cells, but not MA3-positive melanoma cells. (a) MA3/DP4 TCR T cells specifically lyse DP4-positive B cells transduced with MA3-encoding cDNA. Human T cells were tested in a 6 h 51Cr-release assay using EBV-MA3 target cells. The following effector T cells were used: CD4 T-cell clone R12-C9, MA3/DP4 TCR T cells, nondepleted T cells, MA3/DP4 TCR T cells depleted for CD8 T cells, or Mock T cells depleted for CD8 T cells. MA3-negative, DP4-positive B cells (BSM) were not recognized by MA3/DP4 TCR T cells (data not shown). (b) MA3/DP4 TCR T cells do not lyse MZ2-MEL43 melanoma cells, natively expressing MA3 and DP4. Effector T cells used were those described in legend to Figure (a). (c) MA3/DP4 TCR T cells do not lyse MZ2-MEL43 melanoma cells that are pretreated with IFNγ. Target cells were MZ2-MEL43 cells that were either pretreated with IFNγ or not, and effector T cells were MA3/DP4 TCR or Mock T cells. (d) MA3/DP4 TCR T cells lyse MZ2-MEL43 melanoma cells that are pulsed with MA3 peptide. Target cells were MZ2-MEL43 cells that were either pulsed with MA3 peptide or not, and effector T cells were MA3/DP4 TCR T cells. Measurements were performed in triplicate and expressed as mean values corrected for medium values. Data are from representative experiments out of 3 experiments with similar results.
