TCR Gene Transfer: MAGE-C2/HLA-A2 and MAGE-A3/HLA-DP4 Epitopes as Melanoma-Specific Immune Targets
Figure 7
MA3/DP4 TCR CD4 T cells produce cytokines upon coculture with MA3 protein-loaded autologous dendritic cells. MA3/DP4 TCR, and Mock CD4 T cells were cultured with immature or mature autologous dendritic cells that were either loaded with 25 μg/mL MA3 protein or not. After 4 days, supernatants were harvested and analyzed for cytokine production by cytokine bead arrays. Cytokine production was not detected in case T cells were cultured without dendritic cells (data not shown). Measurements were performed in duplicate and expressed as mean values. Data are from a representative experiment out of 2 experiments with similar results.
