Cytotoxic Activity of Dendritic Cells as a Possible Mechanism of Negative Regulation of T Lymphocytes in Pulmonary Tuberculosis

Table 2

The effects of exogenous IL-10 on phenotypic and functional properties of healthy donor DCs.

	N	LPS	LPS + IL-10	P value
B7-H1^+DCs (%)	14	40.4±4.1	48.1±5.3	0.012
Allostimulatory activity in MLC (cpm)	14	13,432 ± 1,841	9,230±992	0.011
Index of stimulation in MLC	14	35.6±6.9	23.7±3.8	0.012
Apoptosis of CD3^+CD4^+ cells in MLC (%)	14	8.3±1.8	10.4±2.3	0.2
Apoptosis of CD3^+CD8^+ cells in MLC (%)	14	11.2±2.0	17.0±2.8	0.006
CD3^+CD4^+ cells in S and G2/M phase (%)	14	17.9±3.3	15.8±3.2	0.18
CD3^+CD8^+ cells in S and G2/M phase (%)	14	18.6±2.2	18.9±2.5	0.92

The relative number of B7-H1⁺DCs (M ± S.E.) was higher in the culture of DCs generated in the presence of IL-10. The allostimulatory activity of DCs generated in the presence of IL-10 was lower than that of DCs generated without IL-10. The generation of healthy donor DCs in the presence of IL-10 was accompanied by increasing CD3⁺CD8⁺ cell apoptosis in MLC stimulated by those DCs.