Journal of Immunology Research / 2012 / Article / Fig 5

Research Article

Impact of the NK Cell Receptor LIR-1 (ILT-2/CD85j/LILRB1) on Cytotoxicity against Multiple Myeloma

Figure 5

Blocking of CD85j and CD159a on NK-92 does not increase specific lysis. Blocking assays were performed as 4-hour CRA. NK-92 were preincubated with human serum and treated with the respective mAb (0.1, 1 and 10  g/mL). E : T ratio of 1.25 : 1 was used throughout all experiments with a total of target cells/200  l. Results are shown as means of triplicates of at least three independent experiments. (a/b): F(ab′)2 was added after the last washing step prior to coincubation with target cells and maintained within the medium throughout the whole experimental period. (C/D): Samples without F(ab′)2. In samples classified as “untreated”, neither F(ab′)2 nor Abs were used. SR was always <20% for all cell lines except from MOLP-8, which showed a constantly high SR up to 36%. Statistical analysis was performed by ANOVA and did not show any significant effect of Ab use.
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