Journal of Immunology Research / 2012 / Article / Fig 7

Research Article

Impact of the NK Cell Receptor LIR-1 (ILT-2/CD85j/LILRB1) on Cytotoxicity against Multiple Myeloma

Figure 7

Blocking of LIR-1 and HLA on tumor cells does not lead to increased lysis by NK-92 cells. LIR-1 (CD85j) and HLA class I molecules of the tumor cell lines were blocked as before on the NK-92 cells. After incubation and extended washing, standard CRA were performed. Neither LIR-1 nor HLA blocking showed significant increase of tumor cell lysis. All experiments were performed with the use of F(ab′)2 to prevent ADCC. Statistical analysis performed by ANOVA excluded any significant effect of either isotype or Ab on target cell lysis, compared to untreated samples. Discrete investigation of K562 treatment, performed as a comparison of cumulated values for all Ab-incubated samples with or without F(ab′)2, excluded any influence of additional F(ab′)2 on the results ( ).
652130.fig.007

We are committed to sharing findings related to COVID-19 as quickly and safely as possible. Any author submitting a COVID-19 paper should notify us at help@hindawi.com to ensure their research is fast-tracked and made available on a preprint server as soon as possible. We will be providing unlimited waivers of publication charges for accepted articles related to COVID-19. Sign up here as a reviewer to help fast-track new submissions.