Impact of the NK Cell Receptor LIR-1 (ILT-2/CD85j/LILRB1) on Cytotoxicity against Multiple Myeloma
Blocking of LIR-1 and HLA on tumor cells does not lead to increased lysis by NK-92 cells. LIR-1 (CD85j) and HLA class I molecules of the tumor cell lines were blocked as before on the NK-92 cells. After incubation and extended washing, standard CRA were performed. Neither LIR-1 nor HLA blocking showed significant increase of tumor cell lysis. All experiments were performed with the use of F(ab′)2 to prevent ADCC. Statistical analysis performed by ANOVA excluded any significant effect of either isotype or Ab on target cell lysis, compared to untreated samples. Discrete investigation of K562 treatment, performed as a comparison of cumulated values for all Ab-incubated samples with or without F(ab′)2, excluded any influence of additional F(ab′)2 on the results ().
Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.