Impact of the NK Cell Receptor LIR-1 (ILT-2/CD85j/LILRB1) on Cytotoxicity against Multiple Myeloma
Use of F(ab′)2 after blocking NK-92 receptors seems to balance lysis pattern. Values show the % relative increase or decrease of target cells lysis in a standard 4-hour CRA, calculated as follows: % relative change of specific lysis = [(% specific lysis “antibody”) − (% specific lysis “untreated”)]/[% specific lysis “untreated”]. Though no concentration of the used mAbs showed a significant change in tumor lysis, interesting differences in the resulting lysis-patterns could be detected. (a) Apart from outliers, use of F(ab′)2 evens out mAb effects in the blocking experiments, leading to values that oscillate close to the origin in both directions. (b) Sparing F(ab′)2 decreases specific lysis, predominantly for MOLP-8 and RPMI8226 cells.
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