Figure 3: Regulatory T cells accumulate in the airways of allergic but not tolerant mice. Time course of (a) CD4+CD25+Foxp3+ (Treg) and (b) CD4+CD25+Foxp3 (Teff) cells number in the BAL of allergic and tolerant mice. (c) Frequency and (d) number of CD4+CD25+ lung cells expressing or not Foxp3. Pooled cells from three mice recovered from BAL and lung were stained for CD4, CD25, and Foxp3 and gated in CD4-positive cells. Values in (a) and (b) represent the means ± SEM for groups of three mice and are representative of two experiments. The data in (c) show a representative experiment of two. Significant differences ** , *** are related to tolerant group.