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Figure 5: Regulatory T cells are not the major producer of suppressive cytokines in the BAL. Quantification by ELISA of BAL (a) IL-10, (b) total, and (c) bioactive TGF-β of BALB/c control, allergic and tolerant mice upon 24 h of the last OVA challenge. (d) IL-10, LAP, and IL-5 staining of BAL CD4+Foxp3+ cells from allergic mice. Pooled cells recovered from BAL of five allergic mice were stained for CD4, Foxp3, IL-10, LAP, and IL-5 and gated in CD4-positive cells. Values represent the means ± SEM for groups of five mice and are representative of two experiments. Significant differences * , *** are shown.