Journal of Immunology Research / 2012 / Article / Fig 4

Research Article

Development of Murine Hepatic NK Cells during Ontogeny: Comparison with Spleen NK Cells

Figure 4

The expression level of CD69 on liver NK cells is higher than on spleen NK. Flow cytometry was performed to analyse lymphocytes stained with the indicated antibodies from liver and spleen of B6 mice at E20, the neonatal stage, and at 3 days, 1 week, 3 weeks, 4 weeks, 6 weeks, 8 weeks, and 10 weeks. NK cells (CD3NK1.1+) were gated to analyse the expression of CD69. In each independent experiment, six to seven fetal mouse livers or spleens were put together to acquire enough cells to perform FACS analysis in one experiment, and three independent experiments were performed. In the other groups, there were three mice independently detected for one experiment, and three independent experiments were performed. (a) Each percentage represents the net percentage (%) of cells in the appropriate quadrant. These results are from a single experiment representative of three independent experiments. (b) The percentages of the CD69+ NK cell subset (%) were calculated for the total number of NK cells in the liver and spleen. Data are shown as mean ± SEM from three mice in each group. *** , ** , * compared with the corresponding group.

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