Research Article

Mapping of Minimal Motifs of B-Cell Epitopes on Human Zona Pellucida Glycoprotein-3

Figure 2

(a) SDS-PAGE analysis of expressed P4 and P12–P15 fusion proteins. (b)–(e) Western blotting of GST188-8mer peptides from P4, P6, P9, and P10 peptides detected using sera to r-hZP3a or r-hZP3b. Note: M, prestained protein marker; Con, uninduced total cell proteins as negative control; P4 and P12–46, total cell proteins of expressed P4; P12–P46 8mer peptide fusion proteins.
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