Nucleotide-Binding Oligomerization Domain-1 and -2 Play No Role in Controlling Brucella abortus Infection in Mice
Figure 2
IFN-γ production by spleen cells induced by B. abortus in NOD1, NOD2, and Rip2 KO mice. C57BL/6, NOD1−/−, NOD2−/−, and RIP2−/− mice were infected with 106 CFU of B. abortus S2308, and 2 weeks after infection, spleen cells (1 × 106/well) were stimulated with B. abortus S2308 (MOI 100 : 1) or concanavalin A (5 μg/mL). Supernatants were harvested after 72 h for measuring IFN- levels by ELISA. Statistically significant difference in relation to nonstimulated cells is denoted with #. The significance of differences was compared by ANOVA followed by Tukey’s test ().
