Characterization of Chronic Cutaneous Lesions from TNF-Receptor-1-Deficient Mice Infected by Leishmania major
Figure 1
Lesions and parasite burdens in TNFR1 KO mice with L. major. Mice were infected with metacyclic stationary forms of L. major in footpads. (a) The footpads were measured weekly and the value for uninfected mice was subtracted from each infected footpad to estimate lesion size. (b) Parasite burden in WT and TNFR1 KO mice. Mice were sacrificed at 6 and 15 weeks afterinfection and parasite burden was determined by limiting dilution analysis ( mice per time point). (c) Lesions from WT and TNFR1 KO mice infected with L. major 15 weeks afterinfection. (d–g) Histological aspect of tissues from lesions from WT and TNFR1 KO infected for 6 weeks (d and e) or 15 weeks (f and g), stained with hematoxylin and eosin. (h) Morfometric quantification of inflammatory cells from WT and TNFR1 KO lesions 15 weeks afterinfection ( mice per group). *. Data are from one experiment of three performed independently.
