Characterization of Chronic Cutaneous Lesions from TNF-Receptor-1-Deficient Mice Infected by Leishmania major
Figure 3
TNF-α, IFN-γ, and IL-4 in lesions from WT and TNFR1 KO mice infected with L. major. Mice were infected in both footpads with L. major (). At 24 and 48 h and at 2, 6, and 11 weeks after infection, mice were killed, lesions were harvested, and RT-PCR reactions performed for cytokines. At 15 weeks protein was assayed by ELISA. (a–c) Fold increase in TNF-α, IFN-γ, and IL-4 mRNA, obtained by densitometry of bands in gels, as described in Section 2. (d–f) TNF-α, IFN-γ, and IL-4 protein was determined by ELISA 15 weeks after infection. Data shown are from one experiment representative of two indenpendently performed ones, with four mice per group. *.
