Journal of Immunology Research / 2012 / Article / Fig 3

Research Article

Characterization of Chronic Cutaneous Lesions from TNF-Receptor-1-Deficient Mice Infected by Leishmania major

Figure 3

TNF-α, IFN-γ, and IL-4 in lesions from WT and TNFR1 KO mice infected with L. major. Mice were infected in both footpads with L. major ( ). At 24 and 48 h and at 2, 6, and 11 weeks after infection, mice were killed, lesions were harvested, and RT-PCR reactions performed for cytokines. At 15 weeks protein was assayed by ELISA. (a–c) Fold increase in TNF-α, IFN-γ, and IL-4 mRNA, obtained by densitometry of bands in gels, as described in Section 2. (d–f) TNF-α, IFN-γ, and IL-4 protein was determined by ELISA 15 weeks after infection. Data shown are from one experiment representative of two indenpendently performed ones, with four mice per group. * .
865708.fig.003a
(a)
865708.fig.003b
(b)
865708.fig.003c
(c)
865708.fig.003d
(d)
865708.fig.003e
(e)
865708.fig.003f
(f)

We are committed to sharing findings related to COVID-19 as quickly and safely as possible. Any author submitting a COVID-19 paper should notify us at help@hindawi.com to ensure their research is fast-tracked and made available on a preprint server as soon as possible. We will be providing unlimited waivers of publication charges for accepted articles related to COVID-19. Sign up here as a reviewer to help fast-track new submissions.