Research Article
Characterization of Chronic Cutaneous Lesions from TNF-Receptor-1-Deficient Mice Infected by Leishmania major
Table 1
Primers, temperature, and number of cycles used for RT-PCR.
| Primer | Sequence | Length (bp) |
| HPRT | 58°C 32 cycles | 217 | FW GTT GGA TAC AGG CCA GAC TTT GTT | 546–569 | RV GAT TCA ACT TGC GCT CAT CTT AGG C | 763–739 |
| IL-4 | 60°C 33 cycles | 292 | FW TTT GAA CGA GGT CAC AGG AG | 195–214 | RV TGC TCT TTA GGC TTT CCA GG | 487–468 |
| IFN-γ | 60°C 32 cycles | 202 | FW GGT GAC ATG AAA ATC CTG CAG | 290–310 | RV GCG CTG GAC CTG TGG GTT GTT GAC C | 493–469 |
| TNF-α | 62°C 30 cycles | 300 | FW CGC TCT TCT GTC TAC TGA AC | 293–312 | RV TGT CCC TTG AAG AGA ACC TG | 593–574 |
| CCL2 | 62°C 30 cycles | 488 | FW CCG GAA TTC CAC TCA CCT GCT GCT ACT CAT TCA | 179–205 | RV CCG GAA TTC GGA TTC ACA GAG AGG GAA AAA TGG | 667–644 |
| CCL5 | 54°C 26 cycles | 309 | FW CGC GGA TCC CCA CGT CAA GG A GTA TTT CTA CAC C | 184–209 | RV CGC GAA TCC CTG GTT TCT TGG GTT TGC TGT TG | 493–473 |
| CCL12 | 54°C 29 cycles | 380 | FW GTT CCT GAC TCC TCT AGC TTT C | 11–32 | RV ACG TAA GAG TTT TTG GAA CTC | 391–371 |
| CXCL9 | 54°C 30 cycles | 398 | FW GAT CAA ACC TGC CTA GAT CC | 302–321 | RV GGC TGT GTA GAA CAC AGA GT | 700–681 |
| CXCL10 | 62°C 27 cycles | 383 | FW CGC GGA TCC TGA GCA GAG ATG TCT GAA TC | 250–271 | RV CGC GGA TCC TCG CAC CTC CAC ATA GCT TAC AG | 633–611 |
| CXCL1 | 62°C 34 cycles | 521 | FW CGC GGA T:CC TGG ACC CTG AAG CTC CCT TGG TTC | 226–251 | RV CGC GGA TCC: CGT GCG TGT TGA CCA TAC ATT ATG | 731–708 |
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