Journal of Immunology Research / 2012 / Article / Tab 1

Research Article

Characterization of Chronic Cutaneous Lesions from TNF-Receptor-1-Deficient Mice Infected by Leishmania major

Table 1

Primers, temperature, and number of cycles used for RT-PCR.

PrimerSequenceLength (bp)

HPRT58°C 32 cycles217
FW GTT GGA TAC AGG CCA GAC TTT GTT546–569
RV GAT TCA ACT TGC GCT CAT CTT AGG C763–739

IL-460°C 33 cycles292
FW TTT GAA CGA GGT CAC AGG AG195–214
RV TGC TCT TTA GGC TTT CCA GG487–468

IFN-γ60°C 32 cycles202
FW GGT GAC ATG AAA ATC CTG CAG290–310
RV GCG CTG GAC CTG TGG GTT GTT GAC C493–469

TNF-α62°C 30 cycles300
FW CGC TCT TCT GTC TAC TGA AC293–312
RV TGT CCC TTG AAG AGA ACC TG593–574

CCL262°C 30 cycles488
FW CCG GAA TTC CAC TCA CCT GCT GCT ACT CAT TCA179–205
RV CCG GAA TTC GGA TTC ACA GAG AGG GAA AAA TGG667–644

CCL554°C 26 cycles309
FW CGC GGA TCC CCA CGT CAA GG A GTA TTT CTA CAC C184–209
RV CGC GAA TCC CTG GTT TCT TGG GTT TGC TGT TG493–473

CCL1254°C 29 cycles380
FW GTT CCT GAC TCC TCT AGC TTT C11–32
RV ACG TAA GAG TTT TTG GAA CTC391–371

CXCL954°C 30 cycles398
FW GAT CAA ACC TGC CTA GAT CC302–321
RV GGC TGT GTA GAA CAC AGA GT700–681

CXCL1062°C 27 cycles383
FW CGC GGA TCC TGA GCA GAG ATG TCT GAA TC250–271
RV CGC GGA TCC TCG CAC CTC CAC ATA GCT TAC AG633–611

CXCL162°C 34 cycles521
FW CGC GGA T:CC TGG ACC CTG AAG CTC CCT TGG TTC226–251
RV CGC GGA TCC: CGT GCG TGT TGA CCA TAC ATT ATG731–708

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