Characterization of Chronic Cutaneous Lesions from TNF-Receptor-1-Deficient Mice Infected by Leishmania major

Table 2

Quantification of apoptosis at the site of infection by L. major in WT and TNFR1 KO mice.

	C57BL/6a		TNFR1 KO
	Mean	Standard error	Mean	Standard error
6 weeks	7.04 b	0.72	6.75	1.00
11 weeks	8.15 c	1.01	2.91	0.52

aMice were infected with L. major in the footpad (1×10⁶) and after 6 and 11 weeks of infection animals were sacrificed and the lesions collected. Tissues were stained by TUNNEL, as described in Section 2. Apoptotic nuclei were counted and results expressed as apoptotic nuclei per 100 nuclei. Results represent the mean of 3–6 animals; six sections were counted per mouse.
^b𝑃=0.37 Student’s 𝑡-test.
^c𝑃=0.0005 Student’s 𝑡-test.