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Clinical and Developmental Immunology
Volume 2012, Article ID 904946, 6 pages
http://dx.doi.org/10.1155/2012/904946
Clinical Study

Transcription Expression and Clinical Significance of Dishevelled-3 mRNA and -Catenin mRNA in Pleural Effusions from Patients with Lung Cancer

1Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Shenyang 110001, China
2Center of Biochip, College of Basic Medical Sciences, China Medical University, Shenyang 110001, China

Received 20 October 2011; Revised 12 December 2011; Accepted 19 December 2011

Academic Editor: Hans Wilhelm Nijman

Copyright © 2012 Xiao-Yan Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. To evaluate diagnostic utility of Dishevelled-3 (DVL-3) mRNA and δ-catenin mRNA expression in pleural effusions of patients with lung cancer. Methods. DVL-3 mRNA and δ-catenin mRNA levels were assessed by performing RT-PCR on pleural effusion specimens from patients with lung cancer ( ) and with lung benign disease ( ). Results. The expressions of DVL-3 mRNA and δ-catenin mRNA were significantly higher in malignant than in benign lung disease ( ) and were obviously higher than cytology in adenocarcinoma ( ). In single use, DVL-3 mRNA had the highest specificity (94.1%) and PPV (95.7%), whereas δ-catenin mRNA had the highest sensitivity (92.0%) and NPV (88.5%). When combinations of markers were evaluated together, DVL-3 mRNA and δ-catenin mRNA gave a high-diagnostic performance: sensitivity of 100.0%, NPV of 100.0%, and accuracy of 96.0%, respectively. Conclusion. As molecular markers of detecting pleural micrometastasis, DVL-3 mRNA and δ-catenin mRNA are helpful to diagnose the cancer cells in pleural effusions of patients with lung cancer.