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Clinical and Developmental Immunology
Volume 2012, Article ID 948218, 10 pages
Clinical Study

The Phenotype of Circulating Follicular-Helper T Cells in Patients with Rheumatoid Arthritis Defines CD200 as a Potential Therapeutic Target

1School of Medicine and Pharmacology, Sir Charles Gairdner Hospital, The University of Western Australia, 4th Floor G Block, Hospital Avenue, Nedlands, Perth, WA 6009, Australia
2Nuffield Department of Clinical Medicine, University of Oxford, Old Road Campus, Roosevelt Drive, Henry Wellcome Building for Molecular Physiology, Oxford OX3 7BN, UK
3Nuffield Department of Rheumatology, Orthopaedics and Musculoskeletal Science, Nuffield Orthopaedic Centre, Windmill Road, Oxford OX3 7HE, UK

Received 6 June 2012; Accepted 26 August 2012

Academic Editor: G. Opdenakker

Copyright © 2012 Aron Chakera et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1: Expression of stimulatory receptors on TFH cells in RA Expression of CD134 (OX-40), CD69, HLA-DR and CD95 in patients with RA compared with controls. There were no significant differences between any of the groups (p>0.05). Each spot represents an individual patient. Mean ± SEM is shown.

Supplementary Figure 2: Induction of CD200 on TFH cells is not due to a circulating factor in patients with high CD200 expression. PBMC from healthy subjects were incubated with serum from RA patients with high or low levels of CD200 expression on TFH cells, and CD200 levels assessed at 24 hours, 48 hours or 72 hours. CD200 expression on TFH cells was induced by PHA but not by serum from patients with low or high CD200 expression (p>0.05). Black columns are unstimulated controls and white columns stimulated samples. Columns shown the mean ± SEM and circles the individual values.

Supplementary Figure 3: Increased CD200 expression in vitro following purification and culture. CD200 expression is significantly increased on freshly purified PBMCs-PBMC (F), when compared with whole blood (WB) (p=0.018) or PBMCs that have been rested for 24 hours before analysis-PBMC (R) (p=0.021). Each spot represents an individual subject. Mean ± SEM is shown.

  1. Supplementary Figure 1
  2. Supplementary Figure 2
  3. Supplementary Figure 3