Immune Modulation in Primary Vaccinia virus Zoonotic Human Infections
CD4+ T-cell activation status in PBMCs from individuals infected or not by zoonotic Vaccinia virus. Peripheral blood mononuclear cells (PBMCs) from patients infected or not with zoonotic Vaccinia virus were cultured in the presence of UV-inactivated virus or mock-treated (medium). After 72 hours of stimulus, cells were fixed, counted, and the following parameters were evaluated by flow cytometry using specific mouse anti-human antibodies: (a) percentage of CD4+ T lymphocytes expressing HLA-DR surface activation marker; (b) percentage of CD4+ T lymphocytes expressing CD25 surface activation marker; (c) percentage of CD4+ T lymphocytes expressing CD69 surface activation marker. Statistical significance ( values), based on the median values of each group, is presented on the graphs.
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