Mycoketide: A CD1c-Presented Antigen with Important Implications in Mycobacterial Infection

Table 1

Distribution of pks12 gene in mycobacteria species^a.

Mycobacteria strains	Gene	Amino acid residues	Identity^b (%)	MPM^c
M. tuberculosis H37Rv	Rv 2048c	4151	100	+
H37Ra	MRA_2063	4151	99.9	+
CDC1551	MT2108	4151	99.7	+
F11	TBFG_12085	4151	99.7	n.d.
KZN1435	TBMG_01933	4152	99.4	n.d.
M. bovis AF2122/97	Mbo2074c	4151	99.4	n.d.
BCG Tokyo 172	JTY_2062	4151	99.6	n.d.
BCG Pasteur 1173P2	BCG_2067c	4151	99.6	+
M. africanum GM041182	MAF_20630	4151	99.9	n.d.
M. canetti CIPT140010059	MCAN_20711	4154	99.0	n.d.
M. marinum ATCC BAA-535	MMAR_3025	4187	83.1	n.d.
M. ulcerans Agy99	MUL_2266	4191	82.7	n.d.
M. avium 104	MAV_2450	4171	80.8	+^d
M. avium subsp. paratuberculosis k10	MAP1796c	4170	80.4	n.d.

^aThe criteria for identifying the pks12 gene is that the encoded Pks protein contains two tandem sets of FAS catalytic domains (KS, AT, DH, ER, KR, and ACP) on one polypeptide and that the two AT domains show substrate specificities for methylmalonyl-CoA and malonyl-CoA, respectively (Figure 1(c)). Without any of these, complete mycoketide structure would not be generated. The PKS database (http://www.nii.res.in/nrps-pks.html) is very useful to predict the catalytic domains and substrate specificity of a Pks enzyme.
^bIdentities to the Rv2048c protein in the aminoacid sequences.
^cThe MPM was determined by a bioassay using the CD8-1 T cells (n.d. not determined).
^dThe MPM production was determined with M. avium serovar 4 strain (ATCC35767).