Dextromethorphan Inhibits Activations and Functions in Dendritic Cells

Figure 1

DXM reduces the expression of immunomodulatory cell surface markers in LPS-induced BMDCs. Immature BMDCs were stimulated with 100 ng/mL LPS with or without DXM for 18 h. Control groups were treated with PBS alone. After incubation, the expression of the surface markers CD40, CD80, CD86, MHC class I and MHC class II was analyzed by flow cytometry with fluorescently labeled Abs. The gray-filled area represents staining with an isotype-matched control Ab. The geometric mean fluorescence intensity (GMFI) of LPS or LPS+DXM is indicated. All data are representative of three independent experiments showing similar results.