Table of Contents Author Guidelines Submit a Manuscript
Clinical and Developmental Immunology
Volume 2013, Article ID 132432, 8 pages
http://dx.doi.org/10.1155/2013/132432
Research Article

Haemophilus parasuis Subunit Vaccines Based on Native Proteins with Affinity to Porcine Transferrin Prevent the Expression of Proinflammatory Chemokines and Cytokines in Pigs

1Unidad de Microbiología e Inmunología, Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de León, 24007 León, Spain
2Laboratório de Imunologia e Doenças Infecto-Contagiosas, Faculdade de Agronomia e Medicina Veterinária, Universidade de Passo Fundo, 99052-900 Passo Fundo, RS, Brazil
3Instituto Tecnológico Agrario de Castilla y León (ITACyL), 47071 Valladolid, Spain

Received 3 July 2013; Revised 22 August 2013; Accepted 27 September 2013

Academic Editor: Roy Ambli Dalmo

Copyright © 2013 R. Frandoloso et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The expression of chemokines (CCL-2 and CXCL-8) and cytokines (IL-1α, IL-1β, IL-6, TNF-α, and IL-10) was evaluated by RT-qPCR in colostrum-deprived pigs vaccinated and challenged with Haemophilus parasuis serovar 5. Two vaccines containing native proteins with affinity to porcine transferrin (NPAPTim and NPAPTit) were tested, along with two control groups: one inoculated with PBS instead of antigen (challenge group (CHG)), and another one nonimmunized and noninfected (blank group). The use of NPAPTim and NPAPTit resulted in complete protection against H. parasuis (no clinical signs and/or lesions), and both vaccines were capable of avoiding the expression of the proinflammatory molecules to levels similar to physiological values in blank group. However, overexpression of all proinflammatory molecules was observed in CHG group, mainly in the target infection tissues (brain, lungs, and spleen). High expression of CCL-2, CXCL-8, IL-1α, IL-1β, and IL-6 can be considered one of the characteristics of H. parasuis infection by serovar 5.