Haemophilus parasuis Subunit Vaccines Based on Native Proteins with Affinity to Porcine Transferrin Prevent the Expression of Proinflammatory Chemokines and Cytokines in Pigs

Table 1

Oligonucleotides used in qPCR analysis.

Genes	Sense	Sequences (5′ to 3′)	Product	Efficiency (%)	Reference
IL1-𝛼	→	GTGCTCAAAACGAAGACGAACC	110 bp	99.5±0.5	Duvigneau et al. [30]
	←	CATATTGCCATGCTTTTCCCAGAA
	→	FAM-TGCTGAAGGAGCTGCCTGAGACACCC-BHQ1
IL1-𝛽	→	CCAAAGGCCGCCAAGATATAA	75 bp	100±0.2	Arce et al. [31]
	←	GGACCTCTGGGTATGGCTTTC
	→	FAM-CTGACTTCACCATGGAAGTCCTCTCTCCCTAAG-BHQ2
TLR-4	→	GCCATCGCTGCTAACATCATC	108 bp	99.7±0.2	This study
	←	CTCATACTCAAAGATACACCATCGG
	→	FAM-CAAAAGTCGGAAGGTTATTGTCGTGGTGTC-BHQ1
IL-6	→	CTGGCAGAAAACAACCTGAACC	94 bp	98.6±0.2	Duvigneau et al. [30]
	←	TGATTCTCATCAAGCAGGTCTCC
	→	FAM-TTGAACCCAGATTGGAAGCATCCGTCTTTT-BHQ1
IL-10	→	CGGCGCTGTCATCAATTTCTG	89 bp	100.4±0.5	Duvigneau et al. [30]
	←	CCCCTCTCTTGGAGCTTGCTA
	→	FAM-AGGCACTCTTCACCTCCTCCACGGC-BHQ1
TNF-𝛼	→	GCCCTGGTACGAACCCATCTA	91 bp	99.1±0.3	Arce et al. [31]
	←	CAGATAGTCGGGCAGGTTGATCTC
	→	FAM-CCAGCTGGAGAAGGATGATCGACTCAGT-BHQ2
CCL-2	→	ACCAGCAGCAAGTGTCCTAAAG	92 bp	99.4±0.7	Arce et al. [31]
	←	TCCTGGACCCACTTCTGCTT
	→	FAM-AGCAGTGATCTTCAAGACCATCGCGG-BHQ2
CXCL-8	→	TTCGATGCCAGTGCATAAATA	120 bp	99.8±0.8	Arce et al. [31]
	←	TGACAAGCTTAACAATGATTTCTGAA
	→	FAM-CATTCCACACCTTTCCACCCCAAATTTATC-BHQ2
GAPDH	→	ACATGGCCTCCAAGGAGTAAGA	106 bp	99.2±0.6	Duvigneau et al. [30]
	←	GATCGAGTTGGGGCTGTGACT
	→	FAM-CCACCAACCCCAGCAAGAGCACGC-BHQ1
cyclophilin	→	TGCTTTCACAGAATAATTCCAGGATTTA	77 bp	100.0±0.1	Duvigneau et al. [30]
	←	GACTTGCCACCAGTGCCATTA
	→	FAM-TGCCAGGGTGGTGACTTCACACGCC-BHQ1
𝛽-actin	→	CTCGATCATGAAGTGCGACGT	114 bp	101.0±0.3	Duvigneau et al. [30]
	←	GTGATCTCCTTCTGCATCCTGTC
	→	FAM-ATCAGGAAGGACCTCTACGCCAACACGG-BHQ1