Combinations of TLR Ligands: A Promising Approach in Cancer Immunotherapy

Figure 2

Direct cytotoxicity of TLR ligand combinations towards CRC cell lines. (a) HROC40, (b) HROC60, and (c) HROC69 cells were treated with all possible combinations between R848, LPS, Poly I:C, and Taxol for 72 h in a concentration of 0.01 μM. Antitumoral effects were determined by flow cytometry. Results after treatment with single TLR ligands (0.01 μM) are additionally shown. Untreated cells without TLR ligand were set as 1 and all data are given as X-fold increase. Experiments were performed in duplicate and repeated at least three times. Values are given as mean + SD; 𝑃<0.05 versus control; t-test.

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