The Leech Nervous System: A Valuable Model to Study the Microglia Involvement in Regenerative Processes
Figure 6
Immunostaining of leech central nervous system (CNS) using rabbit polyclonal anti-human C1qBP antibodies (green). Microglial cell nuclei (white) were stained with Hoechst fluorescent dye to observe cell migration. (a) High magnification image of the injury site after 6 hours. Since all microglia nuclei are shown by Hoechst counterstaining (white), images demonstrate that the anti-C1qBP immunostaining selectively enhances some microglial cells. (b) 24 hours following injury, the number of microglial cells is much higher at the lesion site, and stronger positive immunostaining is observed. (b′) No immunostaining was observed using secondary antibodies alone as negative controls. (Reprinted, with permission, from [74]).
