Upregulated MicroRNA-155 Expression in Peripheral Blood Mononuclear Cells and Fibroblast-Like Synoviocytes in Rheumatoid Arthritis

Figure 6

Suppression of RA-FLS proliferation and invasion by miR-155. (a) Cell proliferation was evaluated using ³H-thymidine incorporation assay. Increased level of miR-155 reduced proliferation of RA-FLS, while decreased level of miR-155 enhanced proliferation of RA-FLS compared to NC (𝑛=5, 𝑃<0.05, 525.53 ± 161.21, 6424.70 ± 2659.66, 3368.60 ± 1084.50, resp.). (b) Transwell invasive assay was used to assess the effect of miR-155 on invasion of RA-FLS. Transfection of miR-155 mimic reduced RA-FLS invasion significantly compared to NC (𝑛=3, 𝑃<0.01, 0.31 ± 0.18, 0.76 ± 0.10, resp.), while transfection of miR-155 inhibitor only showed the trend of increased invasion compared to NC (𝑛=3, 𝑃>0.05, 0.88 ± 0.09, 0.76 ± 0.10, resp.). Each experiment was done in three replicates.

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