Lymphoid Progenitor Cells from Childhood Acute Lymphoblastic Leukemia Are Functionally Deficient and Express High Levels of the Transcriptional Repressor Gfi-1
Figure 2
Defective lymphoid-differentiation potential of primitive bone marrow cells in ALL. Progenitor cell fractions were isolated from control and ALL bone marrow (BM) and placed in lymphoid cocultures for 4 wk on MS-5 stromal cells with SCF, FL, IL-7, and IL-15 before flow cytometry analyses. The general culture approach is shown (a). Cell frequencies of recovered B, NK, conventional dendritic, and plasmacytoid dendritic cells from Lin−CD34+CD45RA+ differentiation cultures were further calculated, and their absolute numbers tabulated as yield per input progenitor ((b) and (c)). Highly purified HSC and CLP cells were cultured in the same conditions and the resulting yields were normalized to control values (d). HSC: hematopoietic stem cells; CLP: common lymphoid progenitor.
