PADI4 Haplotypes in Association with RA Mexican Patients, a New Prospect for Antigen Modulation

Table 1

Genotyping strategies for PADI4 polymorphism variants detection.

SNP	Primers	𝑇𝑚 (°C)	Band size (bp)	Restriction enzyme	Recognized sequence	Band size after digestion (bp)
PADI4_89	5′-TCTGCTTTCCCATGTGTCTTG-3′; 5′-AGGACAGAGTGTGTGTGGCTG-3′	61	278	HaeIII	GGCC	G 100, 95, 43, and 40; A 140, 95, and 43
PADI4_90	5′-AAATCCACAGGTTCCTCCACA-3′; 5′-CATCACGAGCTCTTCCACAGG-3′	62	221	MscI	TGGCA	T 154 and 67; C 221
PADI4_92	5′-CCCAACTTTGTCTCCCCAGT-3′; 5′-TTGTGGTTCACTGACTAAGGAT-3′	61	363	MspI	CCGG	G 195, 134, and 34; C 329 and 34

SNP: single nucleotide polymorphism, 𝑇_𝑚: fusion mean temperature, and bp: base pair.