Allergenic Characterization of New Mutant Forms of Pru p 3 as New Immunotherapy Vaccines
Figure 4
(a) Proliferative response of Pru p 3 T cell lines from peach-allergic patients to wild type, mutants (Pru p 3.01, Pru p 3.02, and Pru p 3.03), LPS, and negative control (PBS), as a measure of incorporated radioactivity. Medians (—) and range (bars) were calculated from values for each protein. Significant differences between the antigens and untreated culture (PBS) are indicated. (b) HLA-II restriction specificity of T cell recognition of wild type and the mutant forms. The inhibition percentage of the proliferation of specific TCLs to each protein in the presence of anti-HLA antibodies is represented for patient 2, as an example.
